Sarcosine Oxidase antibody orb344177 from Biorbyt

Product Specs
ItemSarcosine Oxidase antibody
CompanyBiorbyt
Price Pricing Info Supplier Page
Catalog Numberorb344177
Quantity25 µl
ApplicationsDOT, ELISA, IP, WB
Conjugate/TagUnconjugated
PurityAnti-SARCOSINE OXIDASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Sarcosine Oxidase [Microbial]. Cross reactivity against Sarcosine Oxidase from other sources is unknown
TargetSarcosine Oxidase
IsotypeIgG
ImmunogenSarcosine Oxidase [Microbial]
ClonalityPolyclonal
HostGoat
Concentration1.0 mg/ml
FormatLiquid (sterile filtered)
StorageStore vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing

Specifications/Features

Application Notes: Anti-Sarcosine Oxidase Antibody has been tested by dot blot and western blot and is suitable for ELISA assay against 1.0 ug of Sarcosine Oxidase [Microbial] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:12,000 of the reconstitution concentration is suggested for this product.

Application Dilutions: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000

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